Composite

Part:BBa_K4941108:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-12)


ku70UP-ylER-StYida-URA-ku70dw


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4084
    Illegal PstI site found at 6660
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6660
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1553
    Illegal XhoI site found at 1951
    Illegal XhoI site found at 5077
    Illegal XhoI site found at 5110
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4084
    Illegal PstI site found at 6660
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4084
    Illegal PstI site found at 6660
    Illegal NgoMIV site found at 5265
    Illegal AgeI site found at 2430
    Illegal AgeI site found at 4207
    Illegal AgeI site found at 6035
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1399
    Illegal BsaI.rc site found at 814
    Illegal BsaI.rc site found at 2753
    Illegal BsaI.rc site found at 4530
    Illegal SapI site found at 1701
    Illegal SapI.rc site found at 522


Design Notes

none


Source

yarrowia lipolytica

References